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Inflammatory pain
Inflammatory pain
Inflammatory pain includes an acute nociceptive phase and a sustained phase shaped by inflammatory mediators and sensitization. Separating the phases clarifies mechanism.
Formalin provides a biphasic response and supports preventive or therapeutic dosing designs. CFA extends the design to acute and chronic inflammatory pain.
Phase-specific paw-licking and pain behavior can be cross-validated with mechanical and thermal pain, inflammatory molecules and tissue staining.
- 2Distinct phases
- 2Preventive or therapeutic timing
- CFAAcute and chronic pain
Model strategy
Separate acute and sustained inflammatory phases to compare pain behavior.
Match the model and study conditions to candidate mechanism, intervention stage and target tissue.
Formalin
Biphasic pain behavior
CFA
Inflammatory-pain research
Evaluation endpoints
- 01Paw-licking time and counts
- 02Pain behavior across phases
- 03Mechanical and thermal pain
- 04Inflammatory molecules and histology
Representative data
Formalin model | Modeling procedure
Footpad injection of 2% formalin and the resulting pain behavior in mice.
Formalin model | Phase I and Phase II pain behavior readouts
Phase I (0–5 min) directly stimulates nociceptors and Phase II (10–60 min) reflects inflammatory mediator release with peripheral and central sensitization; licking time and writhing counts for both phases.
Technical contact
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